roxburghii Spreng.) belongs to the ethnobotanically important family Combretaceae. Therefore, some herbs, which have been evaluated for antimicrobial activity, may be used to treat a variety of diseases of microbial origin. Herbal drugs and semi-synthetically produced drugs from botanical sources consisting of nearly 78% of the new drugs approved by the FDA between 19. Īccording to World Health Organization (WHO) herbal medicines derived from medicinal plants would be the best source for curing diseases and provide primary health care to about three quarters of population of developing countries. Plant-derived substances have been used from decades owing to their versatile applications in vector borne diseases, fungal infection, diabetes and many more life threatening ailments. There are plenty of reports in the literature about the antibacterial activity of plant extracts. Plants are the producers of various effective secondary metabolites which are the active ingredients of herbal medicines. However, plants, being the inexhaustible source of many drugs, have attracted scientists from ancient times as an alternative source of the commercially available antibiotics. In addition their use is limited by a number of factors such as low potency, poor solubility and several other side effects. Pathogenic bacteria are often showing resistance to antibiotics available in markets for treating bacterial infections due to their indiscriminate uses. Phytochemical screening and FTIR analysis revealed the presence of tannins, alkaloids, R-CH2-OH groups, aromatics and flavonoids in ethanolic leaf extract qualitatively and these compounds could be responsible for antibacterial property of leaf extracts of C. ConclusionsĪll the tested leaf extracts showed promising antibacterial activity against both gram positive and gram negative bacteria. One way ANOVA and Tukey tests statistically justify the data ( p ≤ 0.05). The results of FTIR analysis confirmed the presence of R-CH2-OH groups, aromatics, C-N stretching amine and NH stretching secondary amine. Qualitatively, the ethanol extract contains flavonoids, tannins and alkaloids. Maximum zones of inhibition were found in case of ethanolic extracts in the following order Bacillus licheniformis (MTCC 530) > Pseudomonas aeruginosa (MTCC 2453) > Bacillus subtilis (MTCC 441) >, Pseudomonas fluorescens (MTCC 103) > Bacillus mycoides (MTCC 7343) > Escherichia coli (MTCC 739) > Pseudomonas putida (MTCC 1654) with zone of inhibition of 27.67 ± 0.33 mm diameter in B. One-way analysis of variance (ANOVA) and Tukey tests were performed for statistical justification. All experiments were conducted in triplicate and values were expressed as the mean ± standard deviation. FTIR analysis was performed to identify the general phytochemical groups of compounds in the extract. Preliminary phytochemical screening was done according to standard protocol.
Diameters of the zone of inhibition were compared with standard antibiotics. MethodsĪntibacterial activity was evaluated against seven bacterial strains by determining minimum inhibitory concentration (MIC) and zone of inhibition. The present study aimed at finding antibacterial potential of aqueous and ethanolic leaf extracts of Combretum album.
Recently plants are used in pharmaceutical industries for novel drug preparations because to ensure efficacy and safety as synthetic antibiotics are threatened for their multidrug resistance. Plant derived medicines show significant contributions to mankind in treating infections of pathogenic bacteria.
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